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Summary
This project uses Laminar Optical Tomography to image the depth-resolved functional activity in exposed cortex. LOT is a new technique that uses measurements of scattered light to add a depth-dimension to conventional multi-spectral optical brain imaging. LOT can currently image hemodynamic activity to > 2mm, with 100-200 micron resolution at over 80 frames per second.
So far, LOT has been demonstrated to be able to resolve the oxy– and deoxy-hemoglobin responses in individual vascular compartments in the somatosensory cortex during forepaw stimulation. This was achieved using a unique spatiotemporal linear fitting procedure based on the characteristic temporal behavior of the arteriolar, capillary and venous responses.
Work is currently underway to add depth-resolved voltage and calcium sensitive fluorescent dye imaging to the system to enable 3D studies of neurovascular coupling in the living brain.
Collaborators
Aniruddha Das PhD. Center for Neurobiology and Behavior, Columbia University College of Physicians and Surgeons
Funding
National Institute of Neurological Disorders and Stroke:
R21 NS053684 (PI: Hillman)
R01 NS063226 (PI: Hillman)
Results and Publications
Burgess S. A, Bouchard M. B, Yuan B, Hillman E. M. C. “Simultaneous Multi-Wavelength Laminar Optical Tomography”, Optics Letters, in press (2008).
Hillman E. M. C, Devor A, Bouchard M. B, Dunn A. K, Krauss GW, Skoch J, Bacskai J, Dale A. M, Boas D. A. “Depth-resolved Optical Imaging and Microscopy of Vascular Compartment Dynamics During Somatosensory Stimulation”, NeuroImage, 35(1): 89-104 (2007)
Hillman E. M. C, “Optical Brain Imaging In-vivo: Techniques and Applications from Animal to Man” [Invited Review] J Biomed Opt, 12(5), 051402 (2007). *Also selected to appear in the “Virtual Journal of Biological Physics Research”
Hillman E. M. C, Burgess S. A, Chen B. “Macroscopic molecular optical imaging of rodent brain”, Book Chapter in ‘Molecular Imaging of the Rodent Brain’ (Neuromethods Series), Editor. Bacskai B. J, Ed. In press 2007, Human Press (Springer).
Hillman E. M. C, Devor A, Dunn AK, Boas DA “Laminar Optical Tomography: High-resolution 3D functional imaging of superficial tissues“ [Invited] In: Medical Imaging, SPIE, San Diego, CA (2006)
Hillman E. M. C, Devor A, Boas DA “High-resolution functional optical imaging of living tissues“ [Invited] In: IEEE International Symposium of Biomedical Imaging Medical Imaging (ISBI) Arlington, VA (2006)
Hillman E. M. C, Bouchard M, Devor A, De Crespigny A, Boas D. A. “Functional optical imaging of brain activation: a multi-scale, multi-modality approach” [Invited] IEEE Proceedings of the Life Science Systems and Application Workshop, Bethesda MD, July 2006.
Hillman, E. M. C, Boas, D. A, Dale, A. M, Dunn, A. K, "Laminar Optical Tomography: demonstration of millimeter-scale depth-resolved imaging in turbid media", Optics Letters, 29, (14), 1650-1652 (2004).
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