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Summary
Two-photon microscopy is a highly versatile and exciting new tool for in-vivo functional imaging. We have constructed our own two-photon microscopy system, optimized for high-speed in-vivo functional brain imaging.
Funding and Collaborators:
We are currently working under a 3 year grant from the HFSP (Human Frontiers Science Program) to collaborate with Dr Bruno Cauli at the Laboratoire de Neurobiologie des Processus Adaptatifs at the Université Pierre et Marie Curie, Paris, France. The grant is entitled: In-vivo and In-vitro: cellular and neurochemical mechanisms of somatosensory neurovascular coupling. We are working together using in-vivo optical imaging and microscopy methods, as well as in-vitro brain slice experiments to explore the underlying mechanisms of neurovascular coupling.
This project has also received a generous contribution from Columbia Central Administration Funds.
Results and Publications
Hillman E. M. C, Devor A, Bouchard M. B, Dunn A. K, Krauss GW, Skoch J, Bacskai J, Dale A. M, Boas D. A. “Depth-resolved Optical Imaging and Microscopy of Vascular Compartment Dynamics During Somatosensory Stimulation”, NeuroImage, 35(1): 89-104 (2007)
Hillman E. M. C, “Optical Brain Imaging In-vivo: Techniques and Applications from Animal to Man” [Invited Review] J Biomed Opt, 12(5), 051402 (2007). *Also selected to appear in the “Virtual Journal of Biological Physics Research”
Hillman E. M. C, Burgess S. A, Chen B. “Macroscopic molecular optical imaging of rodent brain”, Book Chapter in ‘Molecular Imaging of the Rodent Brain’ (Neuromethods Series), Editor. Bacskai B. J, Ed. In press 2007, Human Press (Springer).
Bouchard M, Ruvinskaya S, Boas DA and Hillman E. M. C. “Video-rate two-photon microscopy of cortical hemodynamics in-vivo“ In: OSA Biomedical Topical Meetings, OSA Technical Digest, Fort Lauderdale, FL (2006)
Devor A, Tian P, Nishimura N, Teng IC, Hillman E. M. C, Narayanan S. N, Ulbert I, Boas D. A, Kleinfeld D, Dale A. M. “Suppressed neuronal activity and concurrent arteriolar vasoconstriction may explain negative BOLD”, J Neurosci, 27(16): 4452-4459. (2007) Supplementary Data
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